The USGS Maine Cooperative Fish and Wildlife Research Unit is collaborating with faculty and graduate and undergraduate students in wildlife ecology, remote sensing, and computer science at the University of Maine to investigate the efficacy of using a variety of data collection approaches to survey colonial nesting birds in Maine. This study evaluates data collection platforms (plane, unoccupied aerial vehicles (UAV), ground surveys), data types (multi-platform imagery, real-time observations), and data collection protocols (e.g., UAV speed and altitude) for surveying nests and attending birds. The research team is developing protocols to collect imagery with UAVs with minimal disturbance to birds and to manually interpret the images.